The purpose of this study is to highlight the major aspect of NIPAH virus morphology, its internal structure, transmission and preventions in human. It also includes sample collection major consideration measure that should be taken during collection and what form of sample to taken for laboratory investigation of NIPAH virus. NIPAH virus spread from animal the main reservoir are bat it major breakthrough was first in Malaysia in 1999. Currently there is no effective therapy available. It spreads by direct contact with the infected source. However some preventive measure should be taken so that it can be prevented from spreading from human to human and also from animal to human. In this study an attempt has been taken to elaborate the fact of the major site of action of NIPAH virus in the human genome and every major and minor measure that should be taken to minimize it transmission and infection.